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dnam 1 fc  (R&D Systems)


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    Structured Review

    R&D Systems dnam 1 fc
    (A) Display of ULBP1 and CD155 on CombiCells detected by mAbs and NKG2D-Fc, and <t>DNAM-1-Fc,</t> respectively. (B&C) KIR2DL1+ and KIR2DL1-NK-cell degranulation (CD107a upregulation) in response to ULBP1, CD155, and HLA-C*05:01 displayed on CombiCells. (C) Three types of HLA-C*05:01 were tested, all containing P2 (IIDKSGSTV); wild-type, open and dipeptide exchanged. Data from three independent experiments with NK cells from separate donors are shown.
    Dnam 1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+dnam/Recombinant+Human+DNAM-1%2FCD226+Fc+Chimera+Protein%2C+CF/bio_rxiv__64898__2026__03__03__708729-181-14-15
    Average 94 stars, based on 6 article reviews
    dnam 1 fc - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Using peptide-exchange systems to interrogate peptide-specific KIR binding to HLA Class I"

    Article Title: Using peptide-exchange systems to interrogate peptide-specific KIR binding to HLA Class I

    Journal: bioRxiv

    doi: 10.64898/2026.03.03.708729

    (A) Display of ULBP1 and CD155 on CombiCells detected by mAbs and NKG2D-Fc, and DNAM-1-Fc, respectively. (B&C) KIR2DL1+ and KIR2DL1-NK-cell degranulation (CD107a upregulation) in response to ULBP1, CD155, and HLA-C*05:01 displayed on CombiCells. (C) Three types of HLA-C*05:01 were tested, all containing P2 (IIDKSGSTV); wild-type, open and dipeptide exchanged. Data from three independent experiments with NK cells from separate donors are shown.
    Figure Legend Snippet: (A) Display of ULBP1 and CD155 on CombiCells detected by mAbs and NKG2D-Fc, and DNAM-1-Fc, respectively. (B&C) KIR2DL1+ and KIR2DL1-NK-cell degranulation (CD107a upregulation) in response to ULBP1, CD155, and HLA-C*05:01 displayed on CombiCells. (C) Three types of HLA-C*05:01 were tested, all containing P2 (IIDKSGSTV); wild-type, open and dipeptide exchanged. Data from three independent experiments with NK cells from separate donors are shown.

    Techniques Used:

    Related Articles

    Recombinant:

    Article Title: Graft-versus-host disease predicting marker and use thereof
    Article Snippet: Thereafter, Tx25 monoclonal antibody was isolated using MAb Trap kit purchased from Amersham Bioscience (Uppsala, Sweden). .. Recombinant human DNAM-1/Fc chimeric protein and biotinylated anti-human DNAM-1 polyclonal antibody were purchased from R&D System (Minneapolis, Minn.), and ExtrAvidin peroxidase (including a mouse ExtrAvidin peroxidase staining kit) and Fast o-phenylenediamine dihydrochloride (OPD) tablets were purchased from SIGMA-ALDRICH (Saint Louis, Mo.). ..

    Article Title: Graft-versus-host disease predicting marker and use thereof
    Article Snippet: Thereafter, Tx25 monoclonal antibody was isolated using MAb, Trap kit purchased from Amersham Bioscience (Uppsala, Sweden). .. Recombinant human DNAM-1/Fc chimeric protein and biotinylated anti-human DNAM-1 polyclonal antibody were purchased from R&D System (Minneapolis, Minn.), and ExtrAvidin peroxidase (including a mouse ExtrAvidin peroxidase staining kit) and Fast o-phenylenediamine dihydrochloride (OPD) tablets were purchased from SIGMA-ALDRICH (Saint Louis, Mo.). ..

    Article Title: Induction of the Lytic Cycle Sensitizes Epstein-Barr Virus-Infected B Cells to NK Cell Killing That Is Counteracted by Virus-Mediated NK Cell Evasion Mechanisms in the Late Lytic Cycle
    Article Snippet: APC-conjugated and PE-conjugated antibodies to ULBP2/5/6 (165903) and MICA/B (159207) were purchased from R&D Biosystems. .. Recombinant human DNAM-1/CD226 Fc chimera protein (666-DN-050) was also purchased from R&D Biosystems. ..

    Article Title: Elevated soluble CD226 in Takayasu arteritis is useful for differentiation from giant cell arteritis, disease activity assessment, and prognosis prediction
    Article Snippet: .. After washing, recombinant human DNAM-1/CD226 Fc chimera protein (as a standard) (R&D Systems, Minneapolis) and serum samples were added at 100 μL/well and incubated overnight at 4 °C. .. The plates were washed and incubated with human DNAM-1/CD226 biotinylated antibody (R&D Systems) (0.6 μg/mL, 100 μL/well) for 1 hour at room temperature, and then washed.

    Article Title: Elevated soluble CD226 in Takayasu arteritis is useful for differentiation from giant cell arteritis, disease activity assessment, and prognosis prediction
    Article Snippet: .. After washing, recombinant human DNAM-1/CD226 Fc chimera protein (as a standard) (R&D Systems, Minneapolis) and serum samples were added at 100 μL/well and incubated overnight at 4 °C. .. The plates were washed and incubated with human DNAM-1/CD226 biotinylated antibody (R&D Systems) (0.6 μg/mL, 100 μL/well) for 1 hour at room temperature, and then washed.

    Article Title: Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts.
    Article Snippet: CD155 (poliovirus receptor) localizes in cell–matrix adhesions and cell–cell junctions, but its role in the regulation of cell adhesion and cell motility has not been investigated.. We identified a conserved immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain of human CD155a.. The ITIM was tyrosine-phosphorylated upon binding of anti-CD155 monoclonal antibody D171, poliovirus, and DNAM-1 (CD226) to human CD155a, and recruited SH2-domain-containing tyrosine phosphatase-2 (SHP-2).

    Staining:

    Article Title: Graft-versus-host disease predicting marker and use thereof
    Article Snippet: Thereafter, Tx25 monoclonal antibody was isolated using MAb Trap kit purchased from Amersham Bioscience (Uppsala, Sweden). .. Recombinant human DNAM-1/Fc chimeric protein and biotinylated anti-human DNAM-1 polyclonal antibody were purchased from R&D System (Minneapolis, Minn.), and ExtrAvidin peroxidase (including a mouse ExtrAvidin peroxidase staining kit) and Fast o-phenylenediamine dihydrochloride (OPD) tablets were purchased from SIGMA-ALDRICH (Saint Louis, Mo.). ..

    Article Title: Graft-versus-host disease predicting marker and use thereof
    Article Snippet: Thereafter, Tx25 monoclonal antibody was isolated using MAb, Trap kit purchased from Amersham Bioscience (Uppsala, Sweden). .. Recombinant human DNAM-1/Fc chimeric protein and biotinylated anti-human DNAM-1 polyclonal antibody were purchased from R&D System (Minneapolis, Minn.), and ExtrAvidin peroxidase (including a mouse ExtrAvidin peroxidase staining kit) and Fast o-phenylenediamine dihydrochloride (OPD) tablets were purchased from SIGMA-ALDRICH (Saint Louis, Mo.). ..

    Incubation:

    Article Title: Elevated soluble CD226 in Takayasu arteritis is useful for differentiation from giant cell arteritis, disease activity assessment, and prognosis prediction
    Article Snippet: .. After washing, recombinant human DNAM-1/CD226 Fc chimera protein (as a standard) (R&D Systems, Minneapolis) and serum samples were added at 100 μL/well and incubated overnight at 4 °C. .. The plates were washed and incubated with human DNAM-1/CD226 biotinylated antibody (R&D Systems) (0.6 μg/mL, 100 μL/well) for 1 hour at room temperature, and then washed.

    Article Title: Elevated soluble CD226 in Takayasu arteritis is useful for differentiation from giant cell arteritis, disease activity assessment, and prognosis prediction
    Article Snippet: .. After washing, recombinant human DNAM-1/CD226 Fc chimera protein (as a standard) (R&D Systems, Minneapolis) and serum samples were added at 100 μL/well and incubated overnight at 4 °C. .. The plates were washed and incubated with human DNAM-1/CD226 biotinylated antibody (R&D Systems) (0.6 μg/mL, 100 μL/well) for 1 hour at room temperature, and then washed.



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    (A) Display of ULBP1 and CD155 on CombiCells detected by mAbs and NKG2D-Fc, and <t>DNAM-1-Fc,</t> respectively. (B&C) KIR2DL1+ and KIR2DL1-NK-cell degranulation (CD107a upregulation) in response to ULBP1, CD155, and HLA-C*05:01 displayed on CombiCells. (C) Three types of HLA-C*05:01 were tested, all containing P2 (IIDKSGSTV); wild-type, open and dipeptide exchanged. Data from three independent experiments with NK cells from separate donors are shown.
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    Image Search Results


    (A) Display of ULBP1 and CD155 on CombiCells detected by mAbs and NKG2D-Fc, and DNAM-1-Fc, respectively. (B&C) KIR2DL1+ and KIR2DL1-NK-cell degranulation (CD107a upregulation) in response to ULBP1, CD155, and HLA-C*05:01 displayed on CombiCells. (C) Three types of HLA-C*05:01 were tested, all containing P2 (IIDKSGSTV); wild-type, open and dipeptide exchanged. Data from three independent experiments with NK cells from separate donors are shown.

    Journal: bioRxiv

    Article Title: Using peptide-exchange systems to interrogate peptide-specific KIR binding to HLA Class I

    doi: 10.64898/2026.03.03.708729

    Figure Lengend Snippet: (A) Display of ULBP1 and CD155 on CombiCells detected by mAbs and NKG2D-Fc, and DNAM-1-Fc, respectively. (B&C) KIR2DL1+ and KIR2DL1-NK-cell degranulation (CD107a upregulation) in response to ULBP1, CD155, and HLA-C*05:01 displayed on CombiCells. (C) Three types of HLA-C*05:01 were tested, all containing P2 (IIDKSGSTV); wild-type, open and dipeptide exchanged. Data from three independent experiments with NK cells from separate donors are shown.

    Article Snippet: For binding assays, APC-conjugated Fc fusion protein (NKG2D-Fc (R&D Systems, Cat. No: 1299-NK) or DNAM-1-Fc (R&D Systems, Cat. No: # 666-DN) or KIR-Fc (R&D Systems, KIR2DL1-Fc (Cat. No: 1844-KR-050), KIR2DL3-Fc (Cat. No: 2014-KR-050), KIR2DS4-Fc (Cat. No: 1847-KR-050) (3.6 μg mL -1 ) was added at 25 μL per well and incubated for 30 min at 4 °C in the dark.

    Techniques: